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Production of antibacterial agent from streptomyces griseus by using semi solid fermentation
Author
al-Tai, Muhammad Ibrahim Nadir Mahmud
Source
Journal of the Faculty of Medicine Baghdad
Issue
Vol. 51, Issue 1 (30 Jun. 2009), pp.81-83, 3 p.
Publisher
University of Baghdad Faculty of Medicine
Publication Date
2009-06-30
Country of Publication
Iraq
No. of Pages
3
Main Subjects
Topics
Abstract EN
Backround : The Solid state fermentation has several advantage including absence of free water , reduced volume of production media utilized for high products and the relatively low costs of production. Methods : Thirty local isolates of soil obtained from Genetic Engneering and Biotechnology Institute.
Nutrient agar was used to growth strains examination to antibacterial agent and Wheat bran and fish meal were used in combination (0-100 % of each )and divided in 10 gm lost / flask.
Each flask is inoculated with different numbers of Streptomyces spores and incubated for 5 days at 28°C, then the supernet was extracted and were assayed as antibacterial Results : The ability of 30 local isolates of Streptomyces and the standard strain S.griseus for antibacterial production were tested by grow in Muller Helton agar.
The later isolate (standard isolates) showed highest antibacterial activity with inhibitory effect of (30 mm, 24 mm, 20mm, 18mm) compared with local isolates (S1, S2, S3) resectively Conclusion : only two soil isolates (among 30 isolates) and standard isolates of Streptomyces griseus were appears able to produce antibacterial agent under optimum condition.
The optimum condition for the production of antibacterial is solid state fermentation included the use of wheat bran hydrate with distilled water pH = 8 and the optimum hydration ratio was 1 : 3 (w : v) with an inoculum size of 6 x 109 spores / flask (10 gm wheat bran ) and incubation for 5 days at 28°C.
American Psychological Association (APA)
al-Tai, Muhammad Ibrahim Nadir Mahmud. 2009. Production of antibacterial agent from streptomyces griseus by using semi solid fermentation. Journal of the Faculty of Medicine Baghdad،Vol. 51, no. 1, pp.81-83.
https://search.emarefa.net/detail/BIM-326959
Modern Language Association (MLA)
al-Tai, Muhammad Ibrahim Nadir Mahmud. Production of antibacterial agent from streptomyces griseus by using semi solid fermentation. Journal of the Faculty of Medicine Baghdad Vol. 51, no. 1 (2009), pp.81-83.
https://search.emarefa.net/detail/BIM-326959
American Medical Association (AMA)
al-Tai, Muhammad Ibrahim Nadir Mahmud. Production of antibacterial agent from streptomyces griseus by using semi solid fermentation. Journal of the Faculty of Medicine Baghdad. 2009. Vol. 51, no. 1, pp.81-83.
https://search.emarefa.net/detail/BIM-326959
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 83
Record ID
BIM-326959