The effect of glutathione depletor and inducers in the biliary excretion of cadmium and zinc in rats

Abstract EN

The effect of hepatic glutathione (GSH) depletion and enzymes Induction on hepatic glutathione S-transferase (GST’s) activity, biliary excretion of GSH cadmium, zinc was studied in rats.

The GSH depletor Methyl Iodide did not Influence hepatic GST activity but depending on the substrate used, Phenobarbital benzopyeren increased it by 50-60 %, 20-30 % respectively GSH depletor decreased (60%), benzopyrene did not effect Phenobarbital increase (90 %) the transport of GSH into bile.

The biliary excretion of cadmium and zinc was reduced by methyl iodide depletor (52-65 %) and enhanced by Phenobarbital (99- 110) %.

Treatment with benzopyrene did not affect the excretion of zinc into bile ,but decreased that of cadmium, these result do not provide evidence for the role of hepatic GST but support the importance of biliary GSH excretion in the hepatobiliary transport of cadmium & zinc.

It is assumed that Phenobarbital enhance the biliary excretion of metals by increasing the transport of GSH as a chemical complexation (cadmium-glutathione, zinc-glutathion) while the benzopyrene was less effect.