Immuno-histochemistry analysis of Helicobacter pylori antigen in renal biopsy specimens from patients with glomerulonephritis
Joint Authors
Li, Qian
Lin, Xi
Wu, Zhongbiao
He, Lihuan
Wang, Weijun
Cao, Qizhi
Zhang, Jianzhong
Source
Saudi Journal of Kidney Diseases and Transplantation
Issue
Vol. 24, Issue 4 (31 Dec. 2013), pp.751-758, 8 p.
Publisher
Saudi Center for Organ Transplantation
Publication Date
2013-12-31
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Topics
- Patients
- Kidneys
- Immunohistochemistry
- Antigens
- Diseases
- Biopsy
- Glomerulonephritis
- Diagnosis
- Helicobacter pylori
Abstract EN
This study was conducted to investigate the relationship between Helicobacter pylori infection and three varieties of glomerulonephritis.
Renal biopsy specimens from patients with Henoch Schonlein Purpura nephritis (HSPN ; n = 10), membranous nephropathy (MN; n = 9) and lupus nephritis (LN ; n = 27) were studied using immuno-histochemical labeling to clarify the etiological significance of H.
pylori antigen in this disease.
Immuno-histochemical labeling was performed using a mixture of anti-H.
pylori-antibody-positive serum from nine volunteers; a mixture of anti-H.
pylori-antibody-negative serum from nine volunteers was used as control.
Staphylococci protein-A labeled by horseradish peroxidase was used as the second antibody in this study.
A total of 34 of the 48 specimens revealed positive reaction with the anti-H.
pylori-positive serum and five of the 48 specimens revealed positive reaction with the anti-H.
pylori-negative serum.
Positive reaction against anti-H.
pylori-positive serum was seen in 10 / 10 patients with HSPN, six of nine patients with MN and 18 / 27 patients with LN.
Statistical analysis showed that the difference of the positive reaction between anti-H.
pylori-positive and negative sera was significant (χ 2 = 36.318, P = 0.000).
Our study indicates that H.
pylori infection may be associated with the development and / or progression of HSPN, MN and LN.
American Psychological Association (APA)
Li, Qian& Lin, Xi& Wu, Zhongbiao& He, Lihuan& Wang, Weijun& Cao, Qizhi…[et al.]. 2013. Immuno-histochemistry analysis of Helicobacter pylori antigen in renal biopsy specimens from patients with glomerulonephritis. Saudi Journal of Kidney Diseases and Transplantation،Vol. 24, no. 4, pp.751-758.
https://search.emarefa.net/detail/BIM-328660
Modern Language Association (MLA)
Li, Qian…[et al.]. Immuno-histochemistry analysis of Helicobacter pylori antigen in renal biopsy specimens from patients with glomerulonephritis. Saudi Journal of Kidney Diseases and Transplantation Vol. 24, no. 4 (2013), pp.751-758.
https://search.emarefa.net/detail/BIM-328660
American Medical Association (AMA)
Li, Qian& Lin, Xi& Wu, Zhongbiao& He, Lihuan& Wang, Weijun& Cao, Qizhi…[et al.]. Immuno-histochemistry analysis of Helicobacter pylori antigen in renal biopsy specimens from patients with glomerulonephritis. Saudi Journal of Kidney Diseases and Transplantation. 2013. Vol. 24, no. 4, pp.751-758.
https://search.emarefa.net/detail/BIM-328660
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 757-758
Record ID
BIM-328660