Conventional agar-based culture method, and nucleic acid amplification test (NAAT) of the cppB gene for detection of neisseria gonorrhea in pregnant women endocervical swab specimens
Joint Authors
Hassanzadah, Parvin
Mardanah, Jalal
Mutamidifar, Muhammad
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 15, Issue 3 (31 Mar. 2013), pp.207-211, 5 p.
Publisher
Publication Date
2013-03-31
Country of Publication
United Arab Emirates
No. of Pages
5
Main Subjects
Topics
Abstract EN
Background : Neisseria gonorrhea is the etiological agent of the sexually transmitted disease (STD) gonorrhea, and primarily infects the mucous membranes of the urethra, endocervix, pharynx or rectum of females which may result in substantial morbidity.
N.
gonorrhea also causes disseminated infection, with complications that may result in ectopic pregnancy, tubal infertility, chronic pelvic pain or maternal transmission of gonorrhea, and also increases susceptibility to HIV.
Objectives : In the present investigation, we used conventional agar-based culture method, and nucleic acid amplification of CCPB gene for detection of Neisseria gonorrhea in endocervical swabs samples collected from pregnant women studied Patients and Methods : Endocervical swabs specimens for this study were obtained from 1100 pregnant women who presented to Shiraz (Iran) Hospitals from 2009 to 2011.
In the present investigation we used conventional agar-based culture method, and nucleic acid amplification test (NAAT) of CCPB gene for detection of Neisseria gonorrhea in endocervical swabs samples collected from pregnant women studied.
From each pregnant woman two endocervical swabs were taken : one swab placed in tubes containing phosphate buffered saline for Polymerase Chain Reaction, and the other to inoculate on culture media.
Results : Among 1100 endocervical swabs examined, 13 (1.18 %) samples had positive results by polymerase chain reaction (PCR) on Neisseria gonorrhea CCPB gene.
All endocervical swabs culture had negative results for Neisseria gonorrhea.
84 (7 %) of the women had vaginal discharge, in whom PCR on endocervical swabs of these individuals had negative findings.
Conclusions : Nucleic acid amplification tests (NAATs) are very appropriate in detection of infected individuals.
Detection techniques such as NAATs are independent of bacterial viability, and have a potential to limit false negative samples, therefore, in our country, the application of different laboratory diagnosis methods including NAATs with culture as gold standard for determination antimicrobial susceptibility is essential.
American Psychological Association (APA)
Hassanzadah, Parvin& Mardanah, Jalal& Mutamidifar, Muhammad. 2013. Conventional agar-based culture method, and nucleic acid amplification test (NAAT) of the cppB gene for detection of neisseria gonorrhea in pregnant women endocervical swab specimens. Iranian Red Crescent Medical Journal،Vol. 15, no. 3, pp.207-211.
https://search.emarefa.net/detail/BIM-329087
Modern Language Association (MLA)
Hassanzadah, Parvin…[et al.]. Conventional agar-based culture method, and nucleic acid amplification test (NAAT) of the cppB gene for detection of neisseria gonorrhea in pregnant women endocervical swab specimens. Iranian Red Crescent Medical Journal Vol. 15, no. 3 (2013), pp.207-211.
https://search.emarefa.net/detail/BIM-329087
American Medical Association (AMA)
Hassanzadah, Parvin& Mardanah, Jalal& Mutamidifar, Muhammad. Conventional agar-based culture method, and nucleic acid amplification test (NAAT) of the cppB gene for detection of neisseria gonorrhea in pregnant women endocervical swab specimens. Iranian Red Crescent Medical Journal. 2013. Vol. 15, no. 3, pp.207-211.
https://search.emarefa.net/detail/BIM-329087
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 211
Record ID
BIM-329087