The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of shigella sonnei and shigella flexneri in vitro
Joint Authors
Mirnejad, Rida
Vahdati, Ali Rida
Rashidiani, Jamal
Irfani, Muhammad
Piranfar, Vahhab
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 15, Issue 2 (28 Feb. 2013), pp.122-126, 5 p.
Publisher
Publication Date
2013-02-28
Country of Publication
United Arab Emirates
No. of Pages
5
Main Subjects
Topics
Abstract EN
Backgrounds : Shigellosis remains an important public health problem in developing countries with S.
sonnei and S.
flexneri in US, Europe and in Asian countries being of importance.
Objectives : This study evaluates the protective effect of Lactobacillus casei cell-free culture supernatants (CFCS) against multiple drug resistance (MDR) clinical samples of Shigella sonnei and Shigella flexneri in vitro.
Materials and Methods : S.
sonnei and S .flexneri was identified by common microbiological and serological methods.
Antibiogram with 18 antibiotics were tested for 34 positive cultures by disc diffusion method.
The Samples showed considerable resistance to antibiotics.
Antimicrobial effects of CFCS were tested against S.
sonnei and S.
flexneri by agar-well assay and broth micro dilution methods.
In addition, the antimicrobial activity remained active treatment after adjust pH 7, adding Proteinase K and heating for L.
casei.
Results : The results implicate that L.
casei strongly inhibits the development of pathogen samples.
In contrast, via the disc diffusion method 4 out of 18 antibiogram have shown complete resistance against the pathogen samples.
In addition, the natures of antimicrobial properties have been tested in different conditions such as various pH, temperature and presence of proteinase K.
The MIC50 (minimum inhibitory concentration) and MIC90 of CFCS of L.
casei were determined, for S.
sonnei were 2.25 and 10.5, for S .flexneri were 5.25 and 5.25 respectively.
The results have shown a significant resistance pattern by these four antibiotics in this case.
Conclusions : The data indicates that.
L.
casei highly resistant against to antibiotics, heat, Proteinase K and so many activities against MDR Shigella pathogenic strains .
L.
casei is the best probiotics candidate.
American Psychological Association (APA)
Mirnejad, Rida& Vahdati, Ali Rida& Rashidiani, Jamal& Irfani, Muhammad& Piranfar, Vahhab. 2013. The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of shigella sonnei and shigella flexneri in vitro. Iranian Red Crescent Medical Journal،Vol. 15, no. 2, pp.122-126.
https://search.emarefa.net/detail/BIM-329108
Modern Language Association (MLA)
Mirnejad, Rida…[et al.]. The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of shigella sonnei and shigella flexneri in vitro. Iranian Red Crescent Medical Journal Vol. 15, no. 2 (2013), pp.122-126.
https://search.emarefa.net/detail/BIM-329108
American Medical Association (AMA)
Mirnejad, Rida& Vahdati, Ali Rida& Rashidiani, Jamal& Irfani, Muhammad& Piranfar, Vahhab. The antimicrobial effect of lactobacillus casei culture supernatant against multiple drug resistant clinical isolates of shigella sonnei and shigella flexneri in vitro. Iranian Red Crescent Medical Journal. 2013. Vol. 15, no. 2, pp.122-126.
https://search.emarefa.net/detail/BIM-329108
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 126
Record ID
BIM-329108