RAPD and STS markers linked to stripe rust resistance region Lr46 Yr29 in wheat

Abstract EN

The objective of this study was to identify RAPD and STS markers linked to stripe rust resistance region Lr46 / Yr29 in F3 population of wheat by using the bulked sergeant analysis method.

F3 population has been derived from the cross between the stripe rust resistance parent (monogenic line Lr46 / Yr29) and the susceptible parent (Sids-6) and the evaluation to stripe rust resistance was made at adult stage.

Bulk sergeant analysis was used in conjunction with RAPD analysis.

After Mapmaker linkage analysis on the F3 population, a standard maximum likelihood technique was employed to analyze the linkage between Yr29 and the linked marker loci.

The genetic distance between Lr46 / Yr29 gene and RAPD marker (OPB-8280) was 12.8 cM with LOD scores of 6.4.

Two STS primers BE518048 and TC265717 were tested by using different PCR programs with different annealing temperatures to reveal polymorphism between the monogenic line Lr46 / Yr29 and the cultivar Sids-6.

EST- based STS marker BE518048600 was mapped to Lr46 / Yr29 gene by using the bulked segregant analysis (BSA) procedure with the same previous population.

After Mapmaker linkage analysis on the F3 population, a standard maximum likelihood technique was employed to analyze the linkage between Yr29 and the linked marker loci.

The genetic distance between Lr46 / Yr29 and STS marker BE518048 600 was 11.3 cM with LOD scores of 7.

RAPD and STS markers results revealed that Sids-6 has not Yr29 which linked to Lr46, when evaluated to stripe rust resistance at adult stage.

The availability of such RAPD and STS markers should accelerate the introgression of the resistance gene into current wheat cultivars.

It will also facilitate the accumulation of several resistance genes in a target cultivar.