Some applications of high performance liquied chromatography-determination of trimethoprim

Abstract EN

A simple, fast and precise high performance liquid chromatographic method for the assay of trimethoprim in the presence of sulphamethoxazole has been developed.

The procedure is based on using acetonitril : water: acetic acid in the ratio of 30:69.9:0.1 (v : v : v) as a mobile phase, flow rate (1ml / min.) and uv detector (254nm.).

Each analysis required no longer than 10 minutes.

The detector responses were linear in the range of 0.05-120 μg / ml for trimethoprim with a relative standard deviation of 0.49-0.971 % and a relative error of 1-2.5 %.

The proposed method was also applied satisfactorily to the pharmaceutical preparation.