Indirect spectrophotometric method for determination of bromhexine-hydrochloride in pharmaceutical preparations

Abstract EN

A sensitive indirect spectrophotometric method is developed for the determination of bromhexine-HCl in pure and dosage forms.

The method is based on the oxidation of bromhexine-HCl with iron (III) in acidic medium, then reaction of iron (II) with 1,10- phenathroline to produce a ferroin complex which is water-soluble, stable, and has a maximum absorption at 510 nm against the reagent blank with a molar absorptivity of 3.51 × 104 l.mol-1.cm-1.

Beer’s law is valid over the concentration range of 10 to 220 μg bromhexine-HCl in a final volume of 25 ml.

The proposed method has been applied successfully to determine bromhexine-HCl in different pharmaceutical preparations.