Molecular characteristics of zucchini yellow mosaic virus (ZYMV)‎ on between molecular characteristics of two isolates of virus (ZYMV)‎ that infects cucurbits in Riyadh region, Saudi Arabia

Abstract EN

Zucchini yellow mosaic virus flWX) is the most prevalent virus in cucurbits in Saudi Arabia.

Samples of cucumber (Cucumls satirus) and Pumpkin [Cucurbits moschata) that show symptoms of virus Infection were collected during 2009 growing season from field-grown cucumber and pumpkin in Riyadh Saudi Arabia.

Based on reverse transcription polymerase chain reaction (RT-PCR) analysis using one primer pairs, the virus was Identified as strain of ZYHV.

The PCR assay revealed that the two Riyadh isolates, ZYMV1 (cucumber) and ZYMV2 (Pumpkin) were closely related to ZYMV.

Serological and molecular data Indicated that the two Saudi ZTMV isolates were biologically variable and presented a low molecular diversity.

The Gen bank numbers were GU808330.1 and GU058058.1 for ZYMV1 and ZYMV2, respectively.

The nucleotides sequence of the two isolates ZYMV1 and ZYMV2 presented molecular diversity that suggests that they did not share a common origin and they have adapted to their different hosts.