Visible spectrophotometric determination of ganciclovir in its pharmaceutical formulations using quinalizarin reagent

Abstract EN

A simple, rapid and sensitive spectrophotometric method for the determination of Ganciclovir was developed.

The method is based on the proton transfer reaction of drug with quinalizarin reagent in aqueous solution to form violet product which shows maximum absorbance at 560 nm.

Beer's law was obeyed in the concentration range 1-20 μg.mL-1 with an average recovery (accuracy) of 99.02 % and precision (RSD) of less than 2.0 %.

The molar absorbtivity was 8.93 x 103 l.mol-1.cm-1 with LOD of 0.21 μg.mL-1 and LOQ of 0.71 μg.mL -1.

The proposed method was further applied to the determination of the drug in pharmaceutical formulations as an injection and capsule and the results were comparable with the certified values of pharmaceutical formulations