The expression of Matrix metalloproteinase-2 and cyclooxygenase-2 by immunohistochemistry in patients with colorectal carcinoma

Abstract EN

Background : Human colorectal carcinogenesis is a complex, multistep and multigenetic process.

Matrix metalloproteinase-2 and cyclooxygenase-2 are key enzyme in degradation of extracellular material, are over expressed in several epithelia like colorectal adenocarcinoma.

Objectives : This study was designed to detect the expression of matrix metalloproteinase-2 and cyclooxygenase-2 in patients with colorectal carcinoma and their correlation with age, gender, tumor grade and presence or absence of muscle invasion.

Materials and methods : Immunohistochemical staining of MMP-2 and COX-2 was determined in 40 tissue samples from colorectal patients, from teaching laboratories in Baghdad medical city.

In addition twelve apparently normal colorectal autopsies were used as a control group.

Results : The expression of matrix metalloproteinase-2 was detected in 30 of 40 colorectal cancer cases (75 %) while cyclooxygenase-2 was detected in 31 0f 40 colorectal cancer cases (77.5 %).

All normal colorectal specimens showed negative results.

The incidence of both of them was higher for grade II than those in earlier grade.

Conclusion : Cancer cells to have a more aggressive behavior may show an up regulation of tumor cell derived matrix metalloproteinase-2 and cyclooxygenase-2.