Use polymerase chain reaction for molecular determination of phenol degrading bacteria isolated from Shatt al-Basra, Iraq

Abstract EN

Phenol is one of the organic pollutants in various industrial wastewaters especially petrochemical and oil refining.

Biological treatment is one of the considerable choices for removing of phenol from these wastewaters.

Identification of effective microbial species is considered as one of the important priorities for bacteria producting biomass in order to achieve desirable kinetic of biological reactions.

Basic purpose of this research is identification of phenol-degrading Pseudomonas putida by polymerase chain reaction (PCR) that has high speed and specificity.

Amplification gene coding the N fragment in Pseudomonas putida-derived methyl phenol operon (DmpN gene) was used for specific identification of phenol-degrading Pseudomonas putida,According to the results of this study 7 of 10 isolated bacteria Pseudomonas putida showed a 199 bp PCR product by DmpN primers.