Effect of cisplatin drug on sperm characterizes, spermatogenesis and sex hormones levels of male mice Mus musculus L.

Abstract EN

The present study has aimed to investigate the effect of the anticancer drug cisplatin in sperm count, sperm abnormalities, spermatogenesis and sex hormones in male mice were divided into three groups consisting eight animals in each group.

The first group was served as control and received 0.9 % of normal saline.

The second group was received cisplatin (1 mg / kg).

The third group was received cisplatin (2 mg / kg ).

The mice were treated 8 times of cisplatin (i.

p.) for 16 days at intervals of 48 hr between treatments.

The animals were sacrificed after 16 days of last injection.

The sperm count significantly decreased in the treated with two doses (1and 2mg / kg) at ( p < 0.01).

Cisplatin induced tail abnormalities, but a significant increase was seen in the high dose only.

The spermatogonia, primary spermatocyte, germinal epithelial thickness and seminiferous tubular diameter decreased significantly in the both doses, also elongated spermatids decreased significantly in the treated mice with high dose only.

Drug-induced germinal epithelial sloughing and vacuoles in the seminiferous tubules for testis.

Luteinizing hormone (LH) and testosterone levels decreased significantly in male mice with (1 and 2 mg / kg) doses of cisplatin compared with control group, whereas, there was no significant difference in the serum follicle-stimulating hormone (FSH) of male mice treated with two doses of cisplatin.