Effect of room temperature variations on ELISA results

Abstract EN

This study was designed to investigate the effect of room temperature variations on ELISA results.

Sixty serum samples were collected from healthy and patients persons, distributed as three groups; 1st.

group included 27 samples for thyroids hormones, 2nd.group included 23 samples for some fertility hormones and the 3rd.

group included 10 samples for ferritin.

The concentration of each hormone was estimated at 25 °C and compared twice ; once with the standards of the corresponding hormone were measured at 21 °C ; and the other with the standards of the corresponding hormone were measured at 25 °C.

The results showed that there were variations in the concentrations of each hormone at the two different temperatures.

Regarding thyroids and fertility hormones, the optical density of the standards concentrations at 25 °C were higher than these at 21 °C, while in ferritin test, the optical density of the standards concentrations at 25 °C were lower than these at 21 ˚C.

It could be concluded that the variation in room temperature play a role in estimation of the exact concentrations of hormones in stability of other influencing factors.