Determination of some phenols in Tigris River by HPLC

Abstract EN

This study describes the determination of some phenols in four different zones of Tigris river in Iraq including, AL-Krieat, AL-Kadhimiya, AL-Jadiriyah, and AL-Adhamiyah.

The phenolic compounds analyzed were (2,3-dimethylphenol, 4-chlorophenol, 3-nitrophenol and 2,4-dinitrophenol) using reverse phase high performance liquid chromatography (RP-HPLC) with a UV detector on ODS-C18 column(150 × 4.6 mm I.D) and a mobile phase consisted of methanol-water(30 : 70) %(v / v) at pH 5.0, and a column temperature at 30C° with 20 μL injection.

UV detection helps to identify different phenols at wave length at 280 nm with a flow rate at 0.5 ml / min.

The separation time was (< 6) min.The results indicated that the AL-Adhamiyah zone contains the highest concentration of 3-nitrophenol (0.094 μg / mL), while the AL-Jadiriyah zone recorded the lowest concentration of 3-nitrophenol (0.056 μg / mL).On the other hand the other phenolic compounds studied did not record any concentration in four zones of Tigris river.

The values of correlation coefficient and accuracy were calculated for 3-nitrophenol, the graph was linear with very good correlation coefficient (R2 > 0.9999).