Study of immunological response associated with chronic otitis media caused by staphylococcus aureus

Abstract EN

The present study was aimed to investigate mucosal as well as systemic immune responses of chronic ear discharge patients.

About 100 patients were enrolled in a prospective study just to reaffirm the bacterial profile.

The study indicate the most common three bacterial associated with chronic ear discharge were Pseudomonas aeruginosa 46%, Proteus mirabilis 19%, and Staphylococcus aureus 17%.

Surface antigens were prepared from S.

aureus isolates by benzalchonium chloride.

Humoral and cellular immune responses of chronic ear discharge in patients with S.

aureus isolate at both systemic and mucosal compartment were measured.

Total protein serum patients in cases of S.

aureus increased with mean value 66 g\L, serum immunoglobulins (lg) raised up to 35.5, serum albumin came down to 27.84 g\L and mucosal lg was 0.48 g\L.

In case of S.

aureus, ten patients with chronic ear discharge were studied for serum and mucosal specific immunoglobulin.The serum mean value was 336 and mucosal lg titer was 24.

The titer at systemic and mucosal raised up more than normal value.

Phagocytic activity of polymorph nuclear cells was determined by nitroblue tetrazolium reduction test (NBT) at the peripheral blood and local system.

In patients with S.

aureus, the systemic NBT increased to 35.1% and mucosal 40.8% compared with normal subject.

S.

aureus ear infection was characterized by a significant increase of leucocytes Inhibitory factor (LIF) and E-rosette formation at the systemic and mucosal system, the leucocytes inhibitory factor was 51% and 41% at systemic and mucosal, respectively.

E rosette formation was 38.5% at the systemic and 41% at the mucosal system .