Study of the effect of storage on the level of bilirubin and some serum proteins

Abstract EN

Serum total protein (S.T.P.), aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin, and serum total bilirubin are of biological and chemical importance in the diagnosis and follow up in different diseases.

A variety of changes in the concentration of these substances with the time of storage were found with time.

This research studied the possible changes in the concentration of these substances with time.

The results showed a decrease in all the biochemical substances that measured in the research and the amount of decrease depends on the time.

The decrease depends on the chemical type of each parameter.

Aminotransferases enzymes showed the most significant changes with storage are noticed in enzymes.

It was found a negative relationship between the storage time and each of the measured biochemical parameters in serum.

The changes in serum total bilirubin explained by the possible effect of light on the chemical transformation of bilirubin into lumirubin.

Serum proteins, especially enzymes, decrease due to the possible denaturation caused by freezing and thawing processes.

It is recommended that the analysis should be done immediately after blood aspiration.

If the sample delayed then the results should corrected according to the ratios that obtained in this work.