Alkaloids from glaucium aleppicum papaveraceae

Abstract EN

Five alkaloids were isolated from the methanolic extract of the whole plant material of Glaucium aleppicum Boiss.

& Hausskn.

(Papaveraceae) using bioactivity-directed fractionation as part of a search for anticancer leads from medicinal plants of Jordan flora.

Of these two were protopine-type alkaloids [allocryptopine (1) and protopine (2)], two were aporphine-type alkaloids [(+)-corydine (4) and (+)-isocorydine (5)], and (+)- norchelidonine (3).

Compounds 3-5 were new to the species.

The chemical structures of the isolated compounds were elucidated using a set of spectroscopic and spectrometric techniques, principally: MS, 1D-NMR (1H and 13C) and 2D-NMR (COSY and HMQC).

Compounds 1-5 were tested for general cytotoxicity using BST.

(+)- Norchelidonine was found to possess the highest activity with LC50 value of 0.41 μg/mL and hence evaluated for cytotoxicity against a human cancer cell panel.

(+)-Norchelidonine induced antiproliferative activity against human melanoma MV3 and colorectal SW480 cancer cell lines in a dose-dependent manner with IC50 values of 50 and 80 μg/mL, respectively.