Fermentation studies for the production of dibutyl phthalate, an ester bioactive compound from streptomyces albidoflavus MTCC 3662 using low-priced substrates

Abstract EN

Attempts were made to evaluate the limiting nutritional parameters for production of dibutyl phthalate from Streptomyces albidoflavus MTCC 3662.

The compound was found as a broad spectrum bioactive compound that acts as an antimetabolite of proline.

Among the carbon and nitrogen sources tested in batch fermentation, glycerol (1.75 %) and arginine (0.075 %) supported best production.

Arginine was replaced with sodium nitrate (0.025 %) in the later studied due to its low cost, easy availability and as well as for good production capability.

Other limiting nutrients, KH2PO4 0.05 %, NaCl 0.15 % and MgSO4 0.03 % were found best for production.

Effect of trace salts in the basal medium was also tested.

Medium supplementation with casein hydrolysate, jaggery and yeast extract (0.001 %, each) enhanced the production.

Yield enhancement was also observed with Tween-80 (0.2μg / ml).

Optimization of nutritional parameters imparts enhancement of production by 2.4 fold, using low cost substrates.