Optimization for cellulase production by aspergillus niger using saw dust as substrate

Abstract EN

Cellulase are a group of hydrolytic enzymes capable of degrading the most abundant organic polymer, The aim of this work is to throw insightion parameters that more relavent for enzymatic hydrolysis of saw dust by using Aspergilles niger.

Different cultural conditions were examined to assess their effect in optimizing enzyme production.

The saw dust without any pretreated gave 0.073 IU / ml cellulase activity compared with acid pretreated (0.5 N) & alkaline pretreated (0.3 N) saw dust gave 0.05 IU / ml to 0.045 IU / ml cellulose activity respectively.

Optimum pH for cellulose production was between 4 & 5 (0.083 IU / ml).

Optimal cellulase secretion by Aspergillus niger was achieved after 96 hours of growth & temperature at 35°C.