Purification of exotoxin A from locally isolate of Pseudomonas aeruginosa

Abstract EN

Twenty-five isolates of Pseudomonas aeruginosa were isolated from wounds, burns, inflammation of the urinary tract (UTI), sputum and ear infections.

These isolates were found producing exotoxin A as indicated by ELISA test.

Isolates from different infections (burn, wounds and UTI) were selected according to their productivity.

An amount of 0.045, 0.064 and 0.040 mg / ml protein which represent and 23.31, 29.73 and 19.01 ng / ml exotoxin A were produced respectively.

Extoxoxin A was partially purified using precipitation by 90 % ammonium sulphate followed by ion exchange chromatography with DEAE-cellulose then gel filtration chromatography by sepharose 4B was using to detection the molecular weight for the purified toxin was estimated as 65 KD.