Rht-B1b and Rht-D1b analysis of semidwarf wheat cv. uruq produced by induced mutation

Abstract EN

There are many genes associated with dwarfing growth in bread wheat.

Two genes in particularRht-B1b and Rht-D1b were used for identification of dwarfism in many wheat varieties.

In this study PCR technique was used for identification of Rht-B1b and Rht-D1b in parent cv.

Inia-66 and mutant genes in semidwarf cv.

Uruq.

Two sets of primers were used for identification of mutants genes in semidwarf cv.

Uruq and its parent Inia-66 genes.

The results showed that cv.

Uruq contains the dwarf gene Rht-D1 which is absent in the parent cv.Inia-66.

RAPD-PCR performed in order to establish a molecular marker for cv.

Uruq.

A specific band was observed in cv.

Uruq but absent in cv.

Inia-66.

These results proved that Uruq is semidwarf wheat at the molecular level.