Preparation of Diagnostic Monovalent Antisera Against Staphylococcus aureus

Abstract EN

Background : monovalent antibodies have a great values in the diagnosis and monitoring of infectious diseases.

Objective : the present study an effort to prepare diagnostic monovalent antibodies against Staph.

aureus in order to reduce time and cost of diagnosis in bacteriological and biochemical tests.

Methods : diagnostic monovalent antibodies were prepared against Staphylococcus aureus.

Hyper antibodies were obtained by rabbit immunization with whole antigen of Staphylococcus aureus.

Anti-serum was checked for the presence of antibodies that detected by slide agglutination and ELISA tests.

Sera were purified from cross-reacted antibodies by absorption with whole antigens of other bacterial isolates (Staph.

epidermidis, Pseudomonas aeruginosa, Escherichia coli and Proteus) that cross-reacted with Staph.

aureus antibodies.

Antibodies were purified by repeating absorption process, then checked by ELISA test to diagnose presence of cross-reacted antibodies.

Results : specific monovalent antibodies against Staph.

aureus were tested with different bacterial isolates, it was high sensitive and high specific diagnostic properties.

Conclusions : monovalent antibodies could be used for rapid diagnosis instead of classical way of culture and biochemical tests.