Effect of some antibiotics on uropathogenic escherichia coli and detection of some virulence factors

Abstract EN

In this study, three hundred urine samples were obtained from patients from both sexes suffering from urinary tract infections (UTIs), who have attending to Hilla Teaching Hospital, one hundred thirty four (134) male (44.6 %) and one hundred sixty six (166) female (55.3 %) with no age range.

Out of the) 300(urine samples, only (47) (15.7 %) isolates were found to be related to Escherichia coli.

Hemolysin production by E.

coli was studied and it was found that (19) (40.4 %) out of (47) isolate of E.

coli were able to produce hemolysin on blood agar as zone of hemolysis surrounding bacterial colonies, and other isolates of E.

coli (28) (59.6 %) have able to produce siderophore on M9 media.

Also, the antibiotic sensitivity test was studied on (47) isolates of E.

coli and the results show that these isolates were resistance to Clindamycin (97.8 %), (63.8 %) for Cefotaxime and Cefaxime, Norfloxacine (59.5 %), Naldixic acid (51.0 %), Gentamycin (45.4 %) and Amoxillin (44.6 %, and all isolates were sensitive to Amikacin and Nitrofuranation (100 %), (85 %) for Chloramphenicol, (59.5 %) for Streptomycin and Amoxillin (57.4 %)