High performance liquid chromatographic method for the determination of guaifenesin in pharmaceutical syrups and in environmental samples

Abstract EN

A simple, precise, rapid, and accurate reversed – phase high performance liquid chromatographic method has been developed for the determination of guaifenesin in pure from pharmaceutical formulations.

andindustrial effluent.

Chromatography was carried out on supelco L7 reversed- phase column (25cm × 4.6mm), 5 microns, using a mixture of methanol –acetonitrile-water : (80 : 10 : 10 v / v / v) as a mobile phase at a flow rate of 1.0 ml.min-1.

Detection was performed at 254nm at ambient temperature.

The retention time for guaifenesin was found 2.4 minutes.

The calibration curve was linear (r= 0.9998) over a concentration range from 0.08 to 0.8mg / ml.

Limit of detection (LOD) and limit of quantification ( LOQ) were found 6μg / ml and 18μg / ml respectively.

The method was validated for its linearity, precision and accuracy .The proposed method was successfully applied for the determination of guaifenesin in syrups and industrial effluent samples.