Enhancement of growth and control of browning of tissue cultures of guava (psidium guajava L.)‎

Abstract EN

Difficulties reported on tissue culture of guava were mainly in the initiation phase due to the detrimental effect of phenolic browning beside the low multiplication rate and cultures decline in the proliferation phase.

This study aimed to counteract browning and declining and to enhance growth and multiplication of cultures.

The results revealed that better establishment of in vitro cultures of guava was obtained by vertical positioning of shoot explants and illuminated incubation.

Presoaking explants in a solution containing activat-ed charcoal (AC) counteracted the adverse effect of phenolic browning on cultures vigor especially when activated charcoal (AC) was added to media of 2 passages in initiation phase.

Axillary shoot formation was enhanced by a supplement of 160 mg/l adenine sulphate to multiplication media containing 1 mg/l 6-benzyladenine (BA).

Upon addition of 1 mg/l silver nitrate to media, cultures decline was avoided.

Rooting was enhanced by addition of 1 mg/l indol-3-butyric acid (IBA) to the rooting medium.