Evaluation of the biodegradation of 3,4- di chlorobenzoic acid compounds by bacteria isolated from al-Mufrag

Other Title(s)

تقييم عملية تحطيم مركب 3,4- ثاني كلوروبنزيك عن طريق بكتيريا تم عزلها من منطقة المفرق

Dissertant

al-Atawi, Ibtisam Awad

Thesis advisor

al-Tarawinah, Khalid Ahmad Jafar

Comitee Members

al-Zaraini, Wail Ali Hasan
al-Shakanbah, Jumah Muti
al-Harub, Amir

University

Mutah University

Faculty

Faculty of Science

Department

Department of Biological Sciences

University Country

Jordan

Degree

Master

Degree Date

2013

English Abstract

Different substrate concentration of 3, 4-dCBA were tested for biodegradation by Shigella sonnei.

The higher degradation percentage was obtained with 2mM concentration and the maximum degradation percentage of 3, 4-dCBA was accomplished after 20h of incubation.

The effect of temperatures of 25°C, 30°C and 37°C on the biodegradation of 3, 4-dCBA,The optimal degradation occured at 25°C.

The optimal for degradation were 25°C, pH8.

The degradation occur at 200µl inoculums size.

The carbon sources affected the 3, 4-dCBA degradation differently from that on chloride and cell mass production.

Nitrogen sources used increase the degradation activity of the 3, 4-dCBA as well as in the chlorine release from 3, 4-dCBA.

However, the casein show a slight enhancement of the biodegradation.

These cells were carbon starved for two periods ; 24h and 48h.

The 3, 4-dCBA degradation by non starved cells was more helpful than that of 24 h and 48 h starved cells.

optimum conditions were tested together to show the maximal biodegradation percentage, cell growth and there for Cl release.

The adaptation of Shigella sonnei cells was clearly shown to be crucial aspect for better degradation ability.

Main Subjects

Biology

Topics

No. of Pages

70

Table of Contents

Table of contents.

Abstract.

Chapter One : Theoretical background.

Chapter Two : Literature review.

Chapter Three : Materials and methods.

Chapter Four : Results, discussion and recommendation.

References.

American Psychological Association (APA)

al-Atawi, Ibtisam Awad. (2013). Evaluation of the biodegradation of 3,4- di chlorobenzoic acid compounds by bacteria isolated from al-Mufrag. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-346847

Modern Language Association (MLA)

al-Atawi, Ibtisam Awad. Evaluation of the biodegradation of 3,4- di chlorobenzoic acid compounds by bacteria isolated from al-Mufrag. (Master's theses Theses and Dissertations Master). Mutah University. (2013).
https://search.emarefa.net/detail/BIM-346847

American Medical Association (AMA)

al-Atawi, Ibtisam Awad. (2013). Evaluation of the biodegradation of 3,4- di chlorobenzoic acid compounds by bacteria isolated from al-Mufrag. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-346847

Language

English

Data Type

Arab Theses

Record ID

BIM-346847