Immunodiagnosis of Egyptian human fasciolosis gigantica using FAS1 and FAS2 cysteine proteinase antigens
Joint Authors
Rabi, Ibrahim
Muhanna, Nuha Ahmad
Badr, Abir Mahmud
Source
Journal of the Egyptian Society of Parasitology
Issue
Vol. 43, Issue 3 (31 Dec. 2013), pp.787-796, 10 p.
Publisher
The Egyptian Society of Parasitology
Publication Date
2013-12-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Topics
Abstract EN
problems in the world including Egypt.
Immunodiagnostic methods are more applicable for their better sensitivity and specificity than other methods.
The present study was conducted to cysteine proteinase (CP) antigens of F.
gigantica in IgG-ELISA to diagnose human fasciolosis.
IgG-ELISA with 2 cysteine proteinases of 27 kDa (Fasl) and 29 kDa (Fas2), obtained from the regurgitated materials of adult worms, were evaluated using serum samples from 90 Egyptian patients infected with F.
gigantica, 55 patients with other parasitic infections and 50 healthy volunteers.
The diagnostic sensitivity and specificity of Fas1 for detection of F.
gigantica infection were 91.1% and 89.1%, respectively.
The positivity of the assay was 95%.
The positive and negative predicted values were 91% and 86%, respectively.
These data suggest that IgG-ELISA with Fas1 is highly sensitive and specific assay and could be used for the immunodiagnosis of human fasciolosis.
American Psychological Association (APA)
Rabi, Ibrahim& Muhanna, Nuha Ahmad& Badr, Abir Mahmud. 2013. Immunodiagnosis of Egyptian human fasciolosis gigantica using FAS1 and FAS2 cysteine proteinase antigens. Journal of the Egyptian Society of Parasitology،Vol. 43, no. 3, pp.787-796.
https://search.emarefa.net/detail/BIM-347438
Modern Language Association (MLA)
Rabi, Ibrahim…[et al.]. Immunodiagnosis of Egyptian human fasciolosis gigantica using FAS1 and FAS2 cysteine proteinase antigens. Journal of the Egyptian Society of Parasitology Vol. 43, no. 3 (Dec. 2013), pp.787-796.
https://search.emarefa.net/detail/BIM-347438
American Medical Association (AMA)
Rabi, Ibrahim& Muhanna, Nuha Ahmad& Badr, Abir Mahmud. Immunodiagnosis of Egyptian human fasciolosis gigantica using FAS1 and FAS2 cysteine proteinase antigens. Journal of the Egyptian Society of Parasitology. 2013. Vol. 43, no. 3, pp.787-796.
https://search.emarefa.net/detail/BIM-347438
Data Type
Journal Articles
Language
English
Notes
Includes appendicx : p. 796
Record ID
BIM-347438