Evaluating the genotoxicity of rhazya stricta leaves extract by the saccharomyces cerevisiae auxotrophic mutants test

Abstract EN

Dense cell suspensions of Saccharomyces cerevisiae were treated with elevated concentrations of aqueous extracts of the wild plant Rhazya strict a leaves.

Samples were taken at regular intervals in each treatment and assayed for survival percentage and auxotrophic mutants. It was found that the extract has potent lethal and mutagenic activities.

Survival percentage decreased as concentration or time of exposure increased.

Frequency of auxotrophic mutants increased with increase in concentration or exposure time.

Most auxotrophic mutants were amino acid requiring mutants, less abundant were the nitrogen bases requiring ones and come last the vitamins requiring mutants.