Partial purification of gelatinase enzyme from local isolate of Brevibacillus laterosporus
Joint Authors
Hasan, Hamid Ghafuri
Hamzah, Haydar M.
Ali, Sina Muhammad
Source
Issue
Vol. 2006, Issue 23 (30 Sep. 2006), pp.437-442, 6 p.
Publisher
University of Babylon College of Sciences
Publication Date
2006-09-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract AR
انتج أنزيم الجيلاتينيز من البكتيريا المحلية B.laterosporus باستخدام نظام التخمرات الحالة الصلبة عند استخدام 2:1 وزن/ وزن نخالة الحنطة/ قشور السمك.
كانت فعالية الانزيم بحدود 3.5 وحدة/ مـل لدرجة 37°م عند استخدام المحلول المنظم الترس HCI عند قيمة الاس الهيدروجيني 7.5 .
رسب الأنزيم باستخدام 60 % كبريتات الأمونيوم ثم نقي جزئيا باستخدام كروماتوغرافيا الترشيح الهلامي بعمود السيفادكس باستخدام كروماتوغرافيا الترشيح الهلامي بعمود السيفادكس G-150 و استراد الإنزيم من العمود باستخدام بفر الفوسفيت 0.1 مولار والاس الهيدروجيني 7.4 و تم الحصول على ثلاث قمم ذات فعالية تحليلية للجيلاتين.
و كان عدد مرات التقنية 10.58 و بحصيلة إنزيمية مقدارها 43.42 %.
Abstract EN
A solid state fermentation system consisting of 1:2 w/w wheat bran/fish scales was used for the production of gelatinase enzyme activity.
The activity has been screened from a local isolate of Brevibacillus laterosporus at 37 ºC and using Tris-HCl buffer pH 7.5.
Maximum activity obtained was 3.5 U/ml.
Gelatinase protein content was precipitated using 60% ammonium sulfate.
The enzyme has been partially purified on column chromatography technique using Sephadex G-150 column (1.5X30 cm).
The elution was carried out using buffer phosphate pH 7.4.
Three isoform peaks were recorded from degraded gelatin.
The obtained purification fold and recovery were (10.58) and (43.42) % respectively.
Results indicated the possibility of using purified gelatinase in industry fields.
American Psychological Association (APA)
Hamzah, Haydar M.& Ali, Sina Muhammad& Hasan, Hamid Ghafuri. 2006. Partial purification of gelatinase enzyme from local isolate of Brevibacillus laterosporus. National Journal of Chemistry،Vol. 2006, no. 23, pp.437-442.
https://search.emarefa.net/detail/BIM-348920
Modern Language Association (MLA)
Hasan, Hamid Ghafuri…[et al.]. Partial purification of gelatinase enzyme from local isolate of Brevibacillus laterosporus. National Journal of Chemistry No. 23 (2006), pp.437-442.
https://search.emarefa.net/detail/BIM-348920
American Medical Association (AMA)
Hamzah, Haydar M.& Ali, Sina Muhammad& Hasan, Hamid Ghafuri. Partial purification of gelatinase enzyme from local isolate of Brevibacillus laterosporus. National Journal of Chemistry. 2006. Vol. 2006, no. 23, pp.437-442.
https://search.emarefa.net/detail/BIM-348920
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 441-442
Record ID
BIM-348920