Optimum conditions of 5-nucleotidase activity in sera of kalaazaric patients

Abstract EN

Background : This research focuses on the characterization studies of 5v-Nucleotidase (5'-NT) of kalaazar patients' sera.

5V NT is one of the enzymes used as indicator of liver function test .

In this study the optimum conditions for the activity of 5s-NT enzyme has been demonstrated for kalaazaric patients.

Materials and methods : Thirty five blood samples were obtained from Baghdad Hospitals from untreated infants and children with kala-azar.

5'-NT activity was measured by following the method of Wood and Williams.

Results : Optimum conditions of serum 5'-NT activity of kala- azaric patients obtained were 50 u L , 30 minutes ,0.

8 mM,7.

7 and 50'C) as (Volume of serum, incubation time, substrate concentration, pH and temperature) respectively