DNA content of spermatozoa with respect to seminal sperm concentration using a microchemical spectrophotometric method
Joint Authors
Dawud, Munaf S.
al-Shalabi, Farid A.
Source
Journal of the Faculty of Medicine Baghdad
Issue
Vol. 48, Issue 4 (31 Dec. 2006), pp.410-412, 3 p.
Publisher
University of Baghdad Faculty of Medicine
Publication Date
2006-12-31
Country of Publication
Iraq
No. of Pages
3
Main Subjects
Topics
Abstract EN
Background : To determine the DNA content in subfertile patients and to correlate it with seminal sperm concentration.
Design : Prospective observational study.
Setting : College of Medicine, Dept.
of Physiological chemistry and Institute for embryo Research and infertility treatment-University of Baghdad.The study was conducted through years 2004-2005.
Methods : A random sample of 61 subfertile male patients undergoing semen evaluation and aged from 20-45 years were studied.
Semen samples were assessed for seminal sperm concentration microscopically and were classified into 3 different groups according to count (million / ml).Then sperm D N A content (μg / ml) was estimated using a microchemical spectrophotometric method.
Results: The three groups were statistically of significant difference( P < 0.
01 ).The D N A concentration per spermatozoan in Gr.III ( 1 20 million/ml) was higher than the other two groups It was also noticed that there was a statistically significant (P< 0.05 ) correlation in Gr.III b etween D N A content and sperm concentration .
No significant (P >0.05 )correlation was observed in the other two groups (Gr.
I & Gr.
II ).
Conclusion: Significant positive correlation was obtained between sperm concentration and DNA content.
in the oligozoospermic subfertile patient , but no significant correlation was found in the normozoopermic patients .
D N A content per spermatozoan from oligozoospermic patients was higher than that in normozoospermic patients.
American Psychological Association (APA)
Dawud, Munaf S.& al-Shalabi, Farid A.. 2006. DNA content of spermatozoa with respect to seminal sperm concentration using a microchemical spectrophotometric method. Journal of the Faculty of Medicine Baghdad،Vol. 48, no. 4, pp.410-412.
https://search.emarefa.net/detail/BIM-350011
Modern Language Association (MLA)
Dawud, Munaf S.& al-Shalabi, Farid A.. DNA content of spermatozoa with respect to seminal sperm concentration using a microchemical spectrophotometric method. Journal of the Faculty of Medicine Baghdad Vol. 48, no. 4 (2006), pp.410-412.
https://search.emarefa.net/detail/BIM-350011
American Medical Association (AMA)
Dawud, Munaf S.& al-Shalabi, Farid A.. DNA content of spermatozoa with respect to seminal sperm concentration using a microchemical spectrophotometric method. Journal of the Faculty of Medicine Baghdad. 2006. Vol. 48, no. 4, pp.410-412.
https://search.emarefa.net/detail/BIM-350011
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 412
Record ID
BIM-350011