Vaccination of pregnant guinea pigs with aromatic dependent Salmonella typhimurium to protect their newborns

Abstract EN

Genetically altered stable non reverting aromatic dependent (aro) Salmonella typhimurium,strain SL1479 was administrated intramuscularly to healthy pregnant guinea pigs as alive vaccine.

Twenty one pregnant guinea pigs were divided into two groups, the first group (15 animals) was vaccinated twice with 1ml containing 17 C.f.U /ml approximately fourth and second week preparturition and the second group (6 animals) injected with 1ml trypticase soy broth (TSB) as a control group.

Adverse reaction to vaccination were not observed in the pregnant guinea pigs, which parturated normally.

The vaccine induced humoral and cellular immune response as measured by tube agglutination test and delayed type hypersensitivity(DTH)-skin test in the immunized dams and transfer of this response to the newborns, which revealed a high titers of O(somatic)&H(flagller) agglutination titers and positive delayed type hypersensitivity(DTH)- skin test.

The newborn overcome the challenge with virulent Salmonella typhimurium at 3,6 &8 weeks of age, compared with the control newborn which died.

These results revealed the efficacy of the prenatal vaccination with aro Salmonella typhimurium to transfer the passive immunity to the newborn.