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Ultrastructural and genetic analysis of mucogenicity in klebsiella pneumoniae
Other Title(s)
تحليل تركيبي و وراثي للزوجة بكتريا الكلبسيلا الرئوية
Joint Authors
Radi, Rabab Umran
al-Zaig, Ali Abd al-Rahman
Source
Iraqi Journal of Biotechnology
Issue
Vol. 5, Issue 1 (30 Jun. 2006), pp.85-98, 14 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2006-06-30
Country of Publication
Iraq
No. of Pages
14
Main Subjects
Topics
Abstract AR
A mucoviscous clinical respiratory isolate of K.
pneumoniae was found to produce 414 ng/106 CFU of polysaccharide.
Transmission Electron Microscopy (TEM) revealed that the isolate has a dense thick capsule with a diameter of 190 nm.
The isolate was harboring a single large plasmid encoding multiple resistance to antibiotics.
The isolate was subjected to successive subculturing for 480 generation on M9 medium with and without 150 μg/ml salicylic acid at 37 ºC.
Six mucoid variants were obtained, five (A, B, C, D and E variants) after salicylic acid treatment.
The six derivatives varied in the quantity of polysaccharides produced, genetic and physical behavior and their fine structures as indicated by TEM photography.
One of the variants (variant A) has a small capsule and its colonies lost the mucoviscous phenotype.
Another (variant B) a non-capsulated as indicated by fluorescent antibody staining with antigen serum of wild type.
Plasmid DNA was extracted from each of the six Klebsiella variants and transformed into E.coli MM294 competent cells.
The transformed E.coli showed variable mucoid traits and resistant profile with temperature dependent gene expression of mucogenicity; one of the variants derived plasmid (pKP10) induced mucoid phenotype in E.coli at 37ºC, altered cell division at 37ºC and lead to its inability to ferment lactose on MacConkey medium at both 30 and 37 ºC.
Abstract EN
A mucoviscous clinical respiratory isolate of K.
pneumoniae was found to produce 414 ng / 106 CFU of polysaccharide.
Transmission Electron Microscopy (TEM) revealed that the isolate has a dense thick capsule with a diameter of 190 nm.
The isolate was harboring a single large plasmid encoding multiple resistance to antibiotics.
The isolate was subjected to successive subculturing for 480 generation on M9 medium with and without 150 μg/ml salicylic acid at 37 ºC.
Six mucoid variants were obtained, five (A, B, C, D and E variants) after salicylic acid treatment.
The six derivatives varied in the quantity of polysaccharides produced, genetic and physical behavior and their fine structures as indicated by TEM photography.
One of the variants (variant A) has a small capsule and its colonies lost the mucoviscous phenotype.
Another (variant B) a non-capsulated as indicated by fluorescent antibody staining with antigen serum of wild type.
Plasmid DNA was extracted from each of the six Klebsiella variants and transformed into E.coli MM294 competent cells.
The transformed E.coli showed variable mucoid traits and resistant profile with temperature dependent gene expression of mucogenicity; one of the variants derived plasmid (pKP10) induced mucoid phenotype in E.coli at 37ºC, altered cell division at 37ºC and lead to its inability to ferment lactose on MacConkey medium at both 30 and 37 ºC.
American Psychological Association (APA)
Radi, Rabab Umran& al-Zaig, Ali Abd al-Rahman. 2006. Ultrastructural and genetic analysis of mucogenicity in klebsiella pneumoniae. Iraqi Journal of Biotechnology،Vol. 5, no. 1, pp.85-98.
https://search.emarefa.net/detail/BIM-350437
Modern Language Association (MLA)
Radi, Rabab Umran& al-Zaig, Ali Abd al-Rahman. Ultrastructural and genetic analysis of mucogenicity in klebsiella pneumoniae. Iraqi Journal of Biotechnology Vol. 5, no. 1 (Jun. 2006), pp.85-98.
https://search.emarefa.net/detail/BIM-350437
American Medical Association (AMA)
Radi, Rabab Umran& al-Zaig, Ali Abd al-Rahman. Ultrastructural and genetic analysis of mucogenicity in klebsiella pneumoniae. Iraqi Journal of Biotechnology. 2006. Vol. 5, no. 1, pp.85-98.
https://search.emarefa.net/detail/BIM-350437
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 97-98
Record ID
BIM-350437