Recombinant expression and purification of human placental growth factor 1 and specific camel heavy chain polyclonal antibody preparation

Abstract EN

Placental growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family.

Unlike VEGF, PlGF is dispensable for normal cell development as well as playing various roles in pathological angiogenesis which occurs in tissue ischemia, inflammation, and malignancy.

The PlGF-1 has been considered as a potential candidate for the diagnosis and targeting of pathological angiogenesis.

Camelidae serum contains an important fraction of functional antibodies, called heavy-chain antibodies (HcAbs) that are naturally devoid of light chains.

Camelid HcAbs recognize their cognate antigens by a single variable-domain, referred to as VHH or Nanobody.

Here, we describe the expression and purification of recombinant human PlGF-1 (rhPlGF-1).

This protein was subsequently used for the preparation of camel heavy chain polyclonal antibody against rhPlGF-1.