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Diagnostic value of different antigenic fractions of hydatid cyst fluid from camel and sheep in Kingdom of Saudi Arabia
Joint Authors
al-Ilayyan, Ibtisam M.
Hilmi, Hanan
Source
Journal of Saudi Chemical Society
Issue
Vol. 16, Issue 2 (30 Apr. 2012), pp.203-207, 5 p.
Publisher
Publication Date
2012-04-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
Hydatid cyst fluids (HCF) crude extracts from camels and sheep slaughtered in Riyadhregion, KSA were subjected to Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Western blot analysis.
Sera from 17 confirmed human cases of hydatidosis, 25 patients with other parasitic infections and 10 clinically healthy subjects were used to evaluate the diagnostic value of the different antigenic fractions of these extracts.
Immunoblotting results revealed that, at least 11 major discrete protein fractions (110-8 kDa) were recognized by sera from hydatidosis patients, sera from patients with other parasitic diseases showed cross-reactivity with few of these bands.
The cluster of bands (38-35 kDa) that may be a breakdown of ‘‘Arc 5’’ antigen (39-38 kDa) was detected by 100% and 94% of sera from hydatidosis cases with HCF extracts from camel and sheep, respectively.
This cluster showed also some cross reactivity (20% and 8%) with control sera from patients with other parasitic infections with camel and sheep HCF extracts, respectively.
Polypeptides at 24-22, 16 and 8 kDa which may probably correspond to antigen B subunits were also identified by all samples from hydatidosis patients with sheep HCF extracts and by 100%, 65% and 74% with camel HCF extracts respectively.
Sera from control subjects did not react with any of these polypeptides (24-22, 16 and 8 kDa).
According to our results, the identified molecular weight bands (16 and 8 kDa using HCF crude extracts from sheep and 24-22 kDa using HCF crude extracts either from camel or sheep) represent good candidates for immunodiagnosis of hydatidosis.
American Psychological Association (APA)
al-Ilayyan, Ibtisam M.& Hilmi, Hanan. 2012. Diagnostic value of different antigenic fractions of hydatid cyst fluid from camel and sheep in Kingdom of Saudi Arabia. Journal of Saudi Chemical Society،Vol. 16, no. 2, pp.203-207.
https://search.emarefa.net/detail/BIM-351657
Modern Language Association (MLA)
al-Ilayyan, Ibtisam M.& Hilmi, Hanan. Diagnostic value of different antigenic fractions of hydatid cyst fluid from camel and sheep in Kingdom of Saudi Arabia. Journal of Saudi Chemical Society Vol. 16, no. 2 (Apr. 2012), pp.203-207.
https://search.emarefa.net/detail/BIM-351657
American Medical Association (AMA)
al-Ilayyan, Ibtisam M.& Hilmi, Hanan. Diagnostic value of different antigenic fractions of hydatid cyst fluid from camel and sheep in Kingdom of Saudi Arabia. Journal of Saudi Chemical Society. 2012. Vol. 16, no. 2, pp.203-207.
https://search.emarefa.net/detail/BIM-351657
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 206-207
Record ID
BIM-351657