Comparative analyses of the coat protein of newly discovered ampelovirus using computer-assisted phylogenetic reconstruction and analysis : An approach for classification and characterization

Abstract EN

The coat protein of the newly found closteroviruses Grapevine leaf roll associated vir rus 9 (GLRaV-9) was used for comparative analysis with other members of the family Closteroviridae.

The amino acid alignments using ClustalW demonstrated a significant homology between the GLRaV-9 sequence and the Grapevine leaf roll associated virus 5 (86%) and Pineapple mealybug wilt-associated virus-1 (56%).

GLRaV-9 and GLRaV- 5 were proved to be serologically distinct by using GCG sequence analysis software package that indicate the significant variation (26%) within their polypeptide sequences at the N-terminus of their coat protein.

The phylogenetic analysis, grouped GLRaV-9 with mealybug transmitted viruses (GLRaV-1, -3, -5, and PMWaV-1, -2), suggesting the mode of the transmission of this newly found closterovirus.