An experimental study of collection, separation enumeration and cryopreservation of umbilical cord blood stem cells
Other Title(s)
دراسة تجريبية لجمع و فصل و تعداد و الحفظ بالتجميد للخلايا الجذعية من دم الحبل السري
Joint Authors
Mutlak, Bida Husayn
Mnati, Intidhar Muhammad
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 23, Issue 1 (30 Jun. 2010)8 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2010-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Umbilical cord blood (UCB) contains hematop oietic and mesenchymal stem cells(HSCs,M SCs) that have proven useful clinically to reconstitute the hematop oietic sy stem in children and some adults .
Fifteen cord blood samples were collected from placenta of newly delivered women in Al- Kadhemia hosp ital in Baghdad for normal vaginal delivery.
M ono nucleated cells (M NCs) were isolated by using density gradient centrifugation and the MNCs count and viability were determinated by trypan blue.MNCs were cryopreserved using the cryoprotectant solution of 10% concentration of dimethy l sulfoxid (DM SO)using slow cooling and rapid thaw.
The aim of the present study is to collect, separate and enumerate the stem cells in cord blood(CB) and study the effect of cryopreservation on stem cells count and viability.
The results of the present study showed that the average cord blood volume collected was 81.66 ml.
Mononuclear cell count ranged from 5.6-14.6 x106.Viability count of mononuclear cells was 93.9 %.After three months of cryopreservation , the viability count on revival was 73.9 %.This study represents an attempt to isolate the stem cells from UCB.
The results of the present study confirmed that UCB provides a great source of stem cells for using in medical applications.
American Psychological Association (APA)
Mutlak, Bida Husayn& Mnati, Intidhar Muhammad. 2010. An experimental study of collection, separation enumeration and cryopreservation of umbilical cord blood stem cells. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 23, no. 1.
https://search.emarefa.net/detail/BIM-354105
Modern Language Association (MLA)
Mutlak, Bida Husayn& Mnati, Intidhar Muhammad. An experimental study of collection, separation enumeration and cryopreservation of umbilical cord blood stem cells. Ibn al-Haitham Journal for Pure and Applied Science Vol. 23, no. 1 (2010).
https://search.emarefa.net/detail/BIM-354105
American Medical Association (AMA)
Mutlak, Bida Husayn& Mnati, Intidhar Muhammad. An experimental study of collection, separation enumeration and cryopreservation of umbilical cord blood stem cells. Ibn al-Haitham Journal for Pure and Applied Science. 2010. Vol. 23, no. 1.
https://search.emarefa.net/detail/BIM-354105
Data Type
Journal Articles
Language
English
Notes
Includes appendices.
Record ID
BIM-354105