Molecular cloning of large DNA fragments of the salt tolerant wild tetraploid bermudagrass Cynodon Dactylon L. using a bacteriophage cloning vector : 2. ligation and in vitro packaging of the recombinant phage DNA molecules
Other Title(s)
الكلونة الجزيئية لقطع دنا كبيرة من نبات الثيل البري المقاوم للملوحة باستخدام العاثى كناقل كلونة : 2. ربط و ترزيم جزيئات الدنا المركبة
Author
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 19, Issue 4 (31 Dec. 2006), pp.10-20, 11 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2006-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
This paper represent the second step in a molecular cloning program aiming to clone large DNA fragments ()٢ the salt tolerant berf^udagrass (Cynodon dactylon L.) DNA using the bacteriophage (E.MBL3) as a sector.
In this work, a yield of about 100 ng bacteriophage DNA per one liter culture was obtained with.a purity ranging between (٢١٦٠ م(ة.ا-?.ا vcctor DNA was completely double digested with the restriction enzymes BamHI and FcoRl, followed لاظ purification of vector arms.
Bacteriophage arms were also efficiently ligated with the partially digested bermudagrass DNA (prepared earlier).
The optimum ligation ratio of arms: inserts (EMBL3: bermudagrass) was found to be 4:1 respectively.
The recombinant DNA was successfully in vitro packaged and plated on a P2 lysogen E.
coli (strain NM539).
An efficiency of about 1.3xl0r؛ pfu/ ug recombinant phage DNA was determined ١١؛ these experiments.
American Psychological Association (APA)
Ibrahim, Suhaylah Aid. 2006. Molecular cloning of large DNA fragments of the salt tolerant wild tetraploid bermudagrass Cynodon Dactylon L. using a bacteriophage cloning vector : 2. ligation and in vitro packaging of the recombinant phage DNA molecules. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 19, no. 4, pp.10-20.
https://search.emarefa.net/detail/BIM-355979
Modern Language Association (MLA)
Ibrahim, Suhaylah Aid. Molecular cloning of large DNA fragments of the salt tolerant wild tetraploid bermudagrass Cynodon Dactylon L. using a bacteriophage cloning vector : 2. ligation and in vitro packaging of the recombinant phage DNA molecules. Ibn al-Haitham Journal for Pure and Applied Science Vol. 19, no. 4 (2006), pp.10-20.
https://search.emarefa.net/detail/BIM-355979
American Medical Association (AMA)
Ibrahim, Suhaylah Aid. Molecular cloning of large DNA fragments of the salt tolerant wild tetraploid bermudagrass Cynodon Dactylon L. using a bacteriophage cloning vector : 2. ligation and in vitro packaging of the recombinant phage DNA molecules. Ibn al-Haitham Journal for Pure and Applied Science. 2006. Vol. 19, no. 4, pp.10-20.
https://search.emarefa.net/detail/BIM-355979
Data Type
Journal Articles
Language
English
Notes
Includes appendix : p. 17-19
Record ID
BIM-355979