Isolation and characterization of bacillus licheniformis EM and its antagonistic effect against some important plant pathogens

Abstract EN

The antagonistic action of a newly isolated bacterium derived ٢٠٨١' ; an agricultural waste canal was evaluated against some economic plant pathogens.

The isolate was characterised us, n ^ API 20E commercial kit and molecular identified by partial sequencing of the 16S rDNA.

The phylogenetic analysis strongly related the isolate to Bacillus licheniformis F.

M.

The antimicrobial activity of this isolate was evaluated in vitro against bacteria of crown gall Agrobacterium (umefaciens and soft mold Pectobacterium carolovorum and ; ting؛ of leaf spots and blights Alternaria alternatu.

grey mold Botrytis cinerea, root rot disease Fusarium oxysporurn damping off disease Pythium deharyanum.

The results showed different susceptibility' among th pathogens when serial concentrations 0 licheniformis EM late exponential phase cultures were used.

Out of seven concentrations tested, the most significant antagonistic action was shown by bacterial suspension/20 ml NA medium, which showed almost complete inhibition against the tested bacteria.

All the tested concentrations of licheniformis EM ranged from 20 to 500 Jj.i / 20mi PDA medium completely prevent ft ^ gi of A.

alternata and p.

deharyanum to grow.

Therefore, the study suggests that B.

licheniformis EM can be used as a potential biocontrol agent and recommend it for commercial formulations.

Antagonistic activity; licheniformis EM; Antagonism, Plant