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Evidence of apoptosis in some cell types due to pentachlorophenol (PCP) in heteropneustes fossilis
Joint Authors
Ali, Muhammad Niamat
Nazim, Naziya
Lone, Muhammad Iqbal
Shaykh, Sibhghatulla
Ahmad, Wasim
Source
Saudi Journal of Biological Sciences
Issue
Vol. 20, Issue 1 (31 Jan. 2013), pp.45-49, 5 p.
Publisher
Publication Date
2013-01-31
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
-The study aimed to clarify the role of apoptosis in pentachlorophenol (PCP) induced testicular, ovarian and renal cell genotoxicity of Heteropneustes fossilis.
It was further intended to find the target germ cell type and assess the cellular and nuclear damage.
Treatment of PCP was used for multiduration on the germinal tissues and they were processed to detect structural changes by light and electron microscopic evaluation and kidney cells for subsequent detection of DNA fragmentation by agarose gel electrophoresis.
Findings suggest functional and morphological changes in the tissues are due to apoptosis, as evidenced by some biochemical and cytological signs.
Histological observation on germinal epithelium reveals cell suicidal symptoms such as vacuolization, liquefied regions in the cytoplasm of oocytes, margination of nuclei, clumping of chromatin, and compaction of cytoplasmic organelle.
Biochemical manifestation concurrent to this, is; cleavage of kidney cell DNA into low molecular weight fragments confirming apoptosis.
Subsequently, it is further cleaved into nucleosome size fragments or its multiples.
Ultra-structural histopathology and DNA studies conclusively lead to the PCP induced apoptosis in the exposed cell types.
Results further support the usefulness of this assay in the related studies and its feasibility in generating a base line data.
ª 2012 King Saud University.
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American Psychological Association (APA)
Ali, Muhammad Niamat& Nazim, Naziya& Lone, Muhammad Iqbal& Shaykh, Sibhghatulla& Ahmad, Wasim. 2013. Evidence of apoptosis in some cell types due to pentachlorophenol (PCP) in heteropneustes fossilis. Saudi Journal of Biological Sciences،Vol. 20, no. 1, pp.45-49.
https://search.emarefa.net/detail/BIM-357558
Modern Language Association (MLA)
Ali, Muhammad Niamat…[et al.]. Evidence of apoptosis in some cell types due to pentachlorophenol (PCP) in heteropneustes fossilis. Saudi Journal of Biological Sciences Vol. 20, no. 1 (Jan. 2013), pp.45-49.
https://search.emarefa.net/detail/BIM-357558
American Medical Association (AMA)
Ali, Muhammad Niamat& Nazim, Naziya& Lone, Muhammad Iqbal& Shaykh, Sibhghatulla& Ahmad, Wasim. Evidence of apoptosis in some cell types due to pentachlorophenol (PCP) in heteropneustes fossilis. Saudi Journal of Biological Sciences. 2013. Vol. 20, no. 1, pp.45-49.
https://search.emarefa.net/detail/BIM-357558
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 48-49
Record ID
BIM-357558