Effect of protease and helicase mutations on HCV NS3 activity Zaki Monawar Eisa.
Author
Source
Saudi Journal of Biological Sciences
Issue
Vol. 18, Issue 2 (30 Jun. 2011), pp.195-200, 6 p.
Publisher
Publication Date
2011-06-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Hepatitis C virus (HCV) causes serious infections in the liver which may lead to liver cirrhosis and hepatocellular carcinoma.
Non structural 3 (NS3) protein is one of the most important proteins of the virus which has protease and helicase activities.
Protease activity has a crucial role in the replication and persistence of the virus.
Site directed mutation was carried out in the protease region of one NS3 and another site directed mutation in the helicase region of another NS3.
The expression of both mutated NS3 was compared with wild NS3.
Expression of the three different NS3 types was confirmed by in situ staining and western blotting using an anti-NS3 antibody and correlated with a reduced antiviral response after treatment with interferon-a.
Mutation analysis showed that the NS3 protease activity andnot the NS3 helicase was essential for the inhibition of the interferon-a response.
American Psychological Association (APA)
Isa, Zaki Munawwir. 2011. Effect of protease and helicase mutations on HCV NS3 activity Zaki Monawar Eisa.. Saudi Journal of Biological Sciences،Vol. 18, no. 2, pp.195-200.
https://search.emarefa.net/detail/BIM-357596
Modern Language Association (MLA)
Isa, Zaki Munawwir. Effect of protease and helicase mutations on HCV NS3 activity Zaki Monawar Eisa.. Saudi Journal of Biological Sciences Vol. 18, no. 2 (2011), pp.195-200.
https://search.emarefa.net/detail/BIM-357596
American Medical Association (AMA)
Isa, Zaki Munawwir. Effect of protease and helicase mutations on HCV NS3 activity Zaki Monawar Eisa.. Saudi Journal of Biological Sciences. 2011. Vol. 18, no. 2, pp.195-200.
https://search.emarefa.net/detail/BIM-357596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 199-200
Record ID
BIM-357596