Detection of fusarium wilt pathogens of psidium guajava L. in soil using culture independent PCR (ciPCR)
Joint Authors
Mishra, Rupesh K.
Pandey, Brajesh K.
Muthukumar, M.
Pathak, Neelam
Zeeshan, Muhammad
Source
Saudi Journal of Biological Sciences
Issue
Vol. 20, Issue 1 (31 Jan. 2013), pp.51-56, 6 p.
Publisher
Publication Date
2013-01-31
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Traditional culturing methods take a long time for identification of pathogenic isolates.
A protocol has been developed for the detection of Fusarium from soil samples in the early stage of infection.
Seventeen soil samples from different locations were collected before the onset of rains to find out the presence of Fusarium spp.
population present in the soil of guava orchards and to correlate its presence with incidence of wilt.
A PCR based method was developed for the molecular characterization of Fusarium using Fusarium spp.
specific primer.
DNA extracted by this method was free from protein and other contaminations and the yield was sufficient for PCR amplification.
The primer developed in this study was amplifying 230 bp in all infected samples while not in healthy soil.
The specificity and sensitivity of primer were tested on several Fusarium spp.
and found that this primer was amplifying 106 dilution of the fungal DNA.
The present study facilitates the rapid detection of Fusarium spp.
from infected soil samples of guava collected from different agroclimatic regions in India.
A rapid detection method for pathogens and a diagnostic assay for disease would facilitate an early detection of pathogen and lead to more effective control strategies.
American Psychological Association (APA)
Mishra, Rupesh K.& Pandey, Brajesh K.& Muthukumar, M.& Pathak, Neelam& Zeeshan, Muhammad. 2013. Detection of fusarium wilt pathogens of psidium guajava L. in soil using culture independent PCR (ciPCR). Saudi Journal of Biological Sciences،Vol. 20, no. 1, pp.51-56.
https://search.emarefa.net/detail/BIM-357655
Modern Language Association (MLA)
Pandey, Brajesh K.…[et al.]. Detection of fusarium wilt pathogens of psidium guajava L. in soil using culture independent PCR (ciPCR). Saudi Journal of Biological Sciences Vol. 20, no. 1 (Jan. 2013), pp.51-56.
https://search.emarefa.net/detail/BIM-357655
American Medical Association (AMA)
Mishra, Rupesh K.& Pandey, Brajesh K.& Muthukumar, M.& Pathak, Neelam& Zeeshan, Muhammad. Detection of fusarium wilt pathogens of psidium guajava L. in soil using culture independent PCR (ciPCR). Saudi Journal of Biological Sciences. 2013. Vol. 20, no. 1, pp.51-56.
https://search.emarefa.net/detail/BIM-357655
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 55-56
Record ID
BIM-357655