In vitro antagonism of cotton seedlings fungi and characterization of chitinase isozyme activities in trichoderma harzianum
Joint Authors
Amal, A. Asran
Mahmud, S. M. Mustafa
Sabit, K. K.
al-Banna, O. H.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 17, Issue 2 (30 Apr. 2010), pp.153-157, 5 p.
Publisher
Publication Date
2010-04-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
The antagonistic fungus Trichoderma harzianum is widely recognized as a potential biocontrol agent against several soil-borne plant pathogens.
T.
harzinum is rich source of chitinoltic enzymes.
In vitro screening of 5 isolates of T.
harzinum, one isolate of Chaetomium globosum and one isolate of Conetherium mentance, revealed that all of them had reduced growth area of Macrophomina phaseolina, Fusarium solani and Rhizoctonia solani on PDA medium, significantly.
The inhibition percentage ranged from 77.9 % to 55.9 % for M.
phaseolina and 59.2 % to 40.4 % for R.
solani by T.
harzinum and C.
mentance, respectively.
Inhibition for F.
solani ranged from 76.5 % to 55.7 % by T.
harzinum and C.
globosum, respectively.
Isozyme gel electrophoresis was used to assess chitinase activity secreted by selected isolates of T.
harzinum under different pH degrees and temperatures.
Obtained results indicated that activity of chitinase isozyme produced at 30 °C was higher than 15–20 °C for all tested isolates and activity of chitinase produced by isolates No.
4 and 5 of T.
harzinum at pH (7–7.5) was higher than at pH 6, respectively.
American Psychological Association (APA)
Amal, A. Asran& Mahmud, S. M. Mustafa& Sabit, K. K.& al-Banna, O. H.. 2010. In vitro antagonism of cotton seedlings fungi and characterization of chitinase isozyme activities in trichoderma harzianum. Saudi Journal of Biological Sciences،Vol. 17, no. 2, pp.153-157.
https://search.emarefa.net/detail/BIM-358286
Modern Language Association (MLA)
Amal, A. Asran…[et al.]. In vitro antagonism of cotton seedlings fungi and characterization of chitinase isozyme activities in trichoderma harzianum. Saudi Journal of Biological Sciences Vol. 17, no. 2 (Apr. 2010), pp.153-157.
https://search.emarefa.net/detail/BIM-358286
American Medical Association (AMA)
Amal, A. Asran& Mahmud, S. M. Mustafa& Sabit, K. K.& al-Banna, O. H.. In vitro antagonism of cotton seedlings fungi and characterization of chitinase isozyme activities in trichoderma harzianum. Saudi Journal of Biological Sciences. 2010. Vol. 17, no. 2, pp.153-157.
https://search.emarefa.net/detail/BIM-358286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 157
Record ID
BIM-358286