Multiple molecular markers MAGE-1, MAGE-3 and AFP MRNAs expression nested PCR assay for sensitive and specific detection of circulating hepatoma cells : enhanced detection of hepatocellular carcinoma

Abstract EN

Hepatocellular Carcinoma is a multifactorial, multistep and complex process.

Its prognosis is poor and early diagnosis and monitoring of metastasis of HCC is of utmost importance.

Circulating alpha-fetoprotien mRNA has been proposed as a marker of HCC cells disseminated into the circulation but the specificity of this molecular marker and its correlation with the main HCC clinico-pathological parameters remain controversial.

In recent years ; several different multi-marker assays have been developed for the detection of hepatoma cells in the peripheral blood of patients with HCC.

In this study 58 patients and 15 matched healthy volunteers were included ; the patients were divided into three groups ; group A : patients with primary HCC (n = 32), group B : patients with cirrhosis with no evidence of HCC (n = 12), group C : patients with metastatic cancer in liver (n = 14).

Group D : 15 healthy volunteers age and sex matched.

The staging of HCC was carried out according to the Tumor / Node / Metastasis (TNM) classification.

Peripheral blood samples were obtained from all subjects ; MAGE-1 and MAGE-3 and AFP mRNAs were detected by nested RT-PCR.