Preference of pathogenic bacterial associated infertility in sheep with special reference to campylobacter foetus and its sensitivity to some antimicrobial agents

Abstract EN

A total of 160 ewes were examined in present study having a history of infertility (55 abortions, and 105 repeat breeders).

Samples f0r bacteriological examination including vaginal swabs, faetal fluid, liver and stomach content of aborted faeti, placenta and blood.

All the isolates encountered in this investigation following use of culture on selective media, biochemical reaction and serological tests.

A total of 16 (10 %) isolates identified as being Campylobacter spp.

were recovered, of which 12 isolates from abortion cases with incidence of (21.8 %) and 4 isolates from repeat breeder with incidence of (3.8 %).

Moreover 12 isolates out of the 16 isolates were identified as C.

fetus subsp fetus with incidence of (7.5 %) and 4 isolates as C jejuni with incidence of (2.5 %).

The brucellae isolate was 6 (3.75 %) from only aborted ewes The incidence of Ovine brucella by Rose Bengal plate test, Buffered acidified plate antigen test and Rivanol test were 3.75 %, 3.125 % and 2.5 % respectvely.

These strains were biotyped as Brucella melitensis biovar3.

Salmonellas spp could be isolated from examined samples with percentage of (9.4 %).

Serotypeing of 15 isolates revealed 8 (5.0 %) S.

abortus ovis (04, 12 : c : 1, 6), 5 (3.1 %) S.dublin (Ol, 9, 12 : g, p) and 2 (1.3) S.

enteriditis (Ol, 9, 12 : g, m).

Latex test was as a rapid serological test, revealed 12 isolates of 15 isolates were identified as Salmonella species with incidence of (80 %).

The overall incidence of Listeria monocytogenes isolated was 5 (3.1 %).

including 4 (7.2 %) from abortion cases and 1 (0.95 %) from repeat breeder case.

Antibiotic sensitivity test for Campylobacter fetus subsp fetus was highly sensitive to Chloramephenical, Gentamycin, Ciprofloxacin and Oxytetracycline with incidence (90 %), (80 %), (70 %) and (70 %) respectively, while was resistant to Penicillin, Nalidixic acid, Erythromycin, and Ampcillin with incidence (100 %), (100 %), (80 %) and (70 %) respectively.

The hygienic significant of the bacteria isolates were discussed.