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Bacillus subtillis RTSBA6 6.00, a new strain isolated from gut of helicoverpa armigera (Lepidoptera : Noctuidae) produces chymotrypsin-like proteases
Joint Authors
Shinde, Ashok A.
Shaikh, Faiyaz K.
Padul, Manohar V.
Kachole, Manvendra S.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 19, Issue 3 (30 Sep. 2012), pp.317-323, 7 p.
Publisher
Publication Date
2012-09-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Exploring bacterial communities with proteolytic activity from the gut of the Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) insect pests was the purpose of this study.
As initial efforts to achieve this goal here we report the isolation of new Bacillus subtillis RTSBA6 6.00 strain from the gut of H.
armigera and demonstrated as proteases producer.
Zymographic analysis revealed 12 proteolytic bands with apparent molecular weights ranging from 20 to 185 kDa.
Although some activity was detected at acidic pH, the major activity was observed at slight alkaline pH (7.8).
The optimum temperature was found to be 35 C with complete loss of activity at 70 C.
All proteases were completely inactivated by PMSF (phenylmethylsulfonyl fluoride) and TPCK (Ntosyl- L-phenylalanine chloromethyl ketone), suggesting that proteases secreted by B.
subtillis RTSBA6 6.00 belong to serine proteases class with chymotrypsin-like activity.
The occurrence of protease producing bacterial community in the gut of the H.
armigera advocates its probable assistance to insect in proteinaceous food digestion and adaptation to protease inhibitors of host plan
American Psychological Association (APA)
Shinde, Ashok A.& Shaikh, Faiyaz K.& Padul, Manohar V.& Kachole, Manvendra S.. 2012. Bacillus subtillis RTSBA6 6.00, a new strain isolated from gut of helicoverpa armigera (Lepidoptera : Noctuidae) produces chymotrypsin-like proteases. Saudi Journal of Biological Sciences،Vol. 19, no. 3, pp.317-323.
https://search.emarefa.net/detail/BIM-359454
Modern Language Association (MLA)
Shinde, Ashok A.…[et al.]. Bacillus subtillis RTSBA6 6.00, a new strain isolated from gut of helicoverpa armigera (Lepidoptera : Noctuidae) produces chymotrypsin-like proteases. Saudi Journal of Biological Sciences Vol. 19, no. 3 (2012), pp.317-323.
https://search.emarefa.net/detail/BIM-359454
American Medical Association (AMA)
Shinde, Ashok A.& Shaikh, Faiyaz K.& Padul, Manohar V.& Kachole, Manvendra S.. Bacillus subtillis RTSBA6 6.00, a new strain isolated from gut of helicoverpa armigera (Lepidoptera : Noctuidae) produces chymotrypsin-like proteases. Saudi Journal of Biological Sciences. 2012. Vol. 19, no. 3, pp.317-323.
https://search.emarefa.net/detail/BIM-359454
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 321-323
Record ID
BIM-359454