Radio receptor assay studies of 125 I-testosterone binding to its receptors in benign and malignant human uterine tumors

Abstract EN

A Radio receptor assay procedure was developed for the determination of testosterone receptors in two groups of human uterine tumor patients (benign and malignant).

The optimum conditions of the binding of l25I-testosterone to the receptors were as follows: receptor concentration: (250 [µg protein), tracer testosterone concentration (0.9 ng per 100[µl), time (8 hr), pH (8.0) and temperatures (4 and 25°C) for benign and malignant tumors respectively.

Incubation of unlabeled testosterone and estradiol with uterine tumor tissue homogenate inhibited the ability of 125I-testosterone to bind the uterine tissue homogenate, while progesterone inhibited the binding slightly.

The use of 0.1 M of sodium halides and 0.025 M of divalent salts were shown to cause different effects on the binding in the two groups.