Spectrophotometric determination of methyl dopa by reaction with tyramine and potassium metaperiodate

Abstract EN

A batch and flow injection (FI) spectrophotometric methods have been developed for the determination of methyl dopa in aqueous solution and in pharmaceutical tablets preparation.

The methods are based on the reaction of methyl dopa with tyramine in the presence of potassium metaperiodate as oxidizing agent.

The water soluble orange colour produced was measured at λmax 481 nm.linearity was observed from 0.

5-20 and from 1-50 μg ml-1 methyl dopa with quantification limits of 0.20 and 0.

43 μg- ml-1 methyl dopa by batch and FI procedure respectively.

The effect of chemical and physical parameters have been carefully considered and the proposed procedures were successfully applied to the determination of methyl dopa in tablet pharmaceutical formulation.