Square wave voltammetric method as a tool for determination of cholesterol quantity in human blood serum

Abstract EN

A new method for the determination of Cholesterol in human blood serum based on Square Wave Voltammetry (SWV) has been described.

This method is relay on the decrease in the reduction peak of 4-aminoantipyrine appeared at (+0.004 V) vs.

(Ag/AgC1,3M KCl) as a reference electrode using Phosphate buffer (pH 7.0) as supporting electrolyte, the method is sensitive and rapid.

The procedure was successfully applied to the determination of cholesterol in various blood samples represent different cases such as : Hypothyroidism, Nephrotic syndrome, Obstructive Jaundice & Diabetes Mellitus.

The results have been compared with those obtained from the spectrophotometric method, a good agreement between the two methods has been obtained with correlation coefficient (r = 0.9986).